Taq DNA Polymerase is a simple, specificity PCR reaction mixture. Simply add primers, template, dNTPs, and PCR grade water, the reagent will execute primer extensions and other molecular biology applications.Taq DNA Polymerase contains two components, including the Taq DNA polymerase and 10X PCR buffer.
The Taq DNA Polymerase is purified from E coli., expressing a Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ →3′ DNA polymerase and a 5′ → 3′ exonuclease activity but lacks a 3′ → 5′ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kilo Dalton. Taq DNA polymerase is heat-stable and will synthesize DNA at elevated temperatures from single-stranded templates in the presence of a primer. The Taq DNA Polymerase is recommended for use in routine PCR reactions. The 10X PCR buffer is optimized for high specificity and guarantees minimal by product formation.